Evaluation of the colorimetric cyanmethemoglobin method and the automatical analyser for hemoglobin estimation

ABSTRACT   Hemoglobin estimation by cyanmethemoglobin method was carried out in the present study. The intensity of the color was measured by colorimeter and autoanalyser. The samples were collected from St Isabel’s Hospital, Chennai and estimation of hemoglobin was done in autoanalyser. The remaining sample was transported to Loyola Health Centre, Chennai and then estimation of hemoglobin was performed with colorimeter. The readings obtained from colorimeter and autoanalyser processed statistically and was compared. Results indicates the insignificant variation between the autoanalyser and colorimeter for estimation of Hemoglobin respectively. Autoanalyser detects more anemic cases than colorimetric method. However, in resource poor condition where laboratory cannot afford expensive autoanalyser, colorimeter can be used as a suitable alternative. Keywords: Colorimeter, Autoanalyser, Hemoglobin.