Isolation 3’-end Fragment of Pun1 gene from Capsicum frutescens L. cultivar Cakra Hijau

<p><em>Pun1 </em>gene <a href="file:///C:/Users/Mohamad%20Mostafa/Desktop/Knowledge%20E/In%20Press%20Conferences/ICBS-2015/Source%20files/ICBS-2015/Soure%20papers-ICBS-2015/25.%20Ria%20Reinnata-080117_Roy.doc#_msocom_1">[A1]</a> is the one of candidate gene that responsible to determine pungency in Capsicum. In previous researches, 1 310 bp fragment of 1 671 bp <em>Pun1 </em>gene from<em> Capsicum frutescens </em>L. cv. Cakra Hijau had been isolated. The purpose of this research was to isolate of 3’-<em>end</em> fragment and get full length of <em>Pun1</em> gene from  <em>C. frutescens</em> L. cv. Cakra Hijau. DNA  isolation was done using modified procedure. The method used to isolate the gene was PCR with a pair of primer, <em>forward primer </em>5’-GAA-GGT-GGC-AGA-AGA-ATC-AG-3’ and <em>reverse primer</em> 5’-TTG- TTG ACC-GTA-AAC-TTC-CG- 3’. The result successfully to get 715 bp length DNA fragment. The assembly of this fragment into previous research produced a full length of 1 671 bp <em>Pun1</em> gene from <em>C. frutescens</em> L. cv. Cakra Hijau consist of 738 bp first exon fragment, 348 bp intron fragment, and 585 bp second exon fragment.</p><p class="Els-Abstract-text"> </p><div><p class="Els-keywords"><strong>Keywords:</strong> C. frustescens L. cv. Cakra Hijau; gene isolation; <em>Pun1</em> gene.</p></div>