ELEKTROCHEMICZNE I BIOLOGICZNE BADANIA DNA: PLAZMIDY I CHROMOSOMALNE DNA KRÓTKA HISTORIA BADAŃ DNA W PRACOWNI ELEKTROANALIZY CHEMICZNEJ

For a number of years, the DNA interactions with various compounds have been a subject of research at the Laboratory of Electroanalytical Chemistry. Plasmids and chromosomal DNAs have been used to investigate and distinguish between the DNA interactions with intercalators, newly synthesized potential anticancer drugs and antioxidants. So far, plasmids have been rarely used in electrochemical experiments and therefore their use can be considered a novelty in our investigations, realized in collaboration with the Molecular Biology Laboratory at the Faculty of Biology of the University of Warsaw. New potential anticancer drugs have been synthesized at the Institute of Organic Industrial Chemistry and tested on cancer cell lines at the Pharmacy Department of the Warsaw Medical University. The methodology of DNA-drugs investigations has been tested with the use of a typical DNA intercalator, Methylene Blue, and resulted in the sequential use of four electrochemical methods, i.e. SW, DP, AC 00 i AC 900 to assess redox properties and capacity/resistance changes of the electrode layers formed. Such complex electroanalytical methodology allowed for distinguishing between the anticancer properties of two seemingly similar compounds, IPBD and Cl-IPBD, which differed only by one substituent. The comparison of these electrochemical results with biological testing (on cancer cell lines) these prospective anticancer drugs, showed good correlation of the two different methods used, in prediction of anticancer properties of new drugs. Thus, it has been shown that the combination of electroanalytical methods can be successfully used for preliminary testing of anticancer drugs, before further confirming their use by complex and time consuming biological methods. It should be noted that the interactions of plasmids with the antioxidants such as: riboflavin (Rb, Wit.B2), Witamin C and Rutin (RU), used in this work and tested by electroanalytical methods, showed contrasting results to the ones obtained for anticancer drugs.