IN VITRO ASSESSMENT OF ANTIOXIDANT AND ANTIMICROBIAL POTENTIAL OF ALANGIUM CHINENSE (LOUR.) HARMS EXTRACT

Objective: The research intended to perform preliminary phytochemical analysis, total phenolic and flavonoid estimation, high-performance thin-layer chromatography (HPTLC) fingerprinting, bioactive marker study, and assays to assess antioxidant powers and determine antimicrobial capacity through zone of inhibition (ZOI) and minimum inhibitory concentration (MIC). This confirmed the antimicrobial and antioxidant properties of Alangium chinense. Methods: Current research work comprised HPTLC fingerprinting bioactive marker study of A. chinense ethanolic extract with TPC and TFC quantitative estimation and In-Vitro of assessment of with 2,20-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl, Hydroxyl radical Scavenging assays, Reactive Nitrogen oxide, Superoxide anion and CUPRAC to determine antioxidant activity followed by determination of antimicrobial activity with 3 Gram-positive bacteria, 2 Gram-negative bacteria and 2 fungal strains includes ZOI and MIC in results. Results and Conclusion: Chemical fingerprinting by HPTLC identified seven major phytoconstituents and verified the presence of rutin, gallic acid, quercetin, and kaempferol, with gallic acid being the most abundant compound. These findings provide scientific evidence for the phytochemical richness of the extract and its potential therapeutic applications. The result shows that highest antioxidant activity for the FRAP assay with a half-maximal inhibitory concentration (IC50) value of 1.97 and the lowest antioxidant activity for the Reactive Nitrogen Oxide radical scavenging assay with an IC50 value of 14.09. Total phenolic content and total flavonoid content estimation is 137.36±3.15 mg GAE/g and 319.64±0.80 mg QE/g extract boosts antioxidant potential. In antimicrobial activity, ZOI range for all strains is between 11.0±1.0 mm and 19.0±1.0 mm. MIC and Non-inhibitory concentration (NIC) result shows Malassezia furfur, Candida albicans, and Serratia marcescens shows potent MIC at 83.92 μg/mL, 109.7 μg/mL, 150.8 μg/mL and NIC at 29.67 μg/mL, 12.32 μg/mL, 13.34 μg/mL with respect to the positive control. Staphylococcus aureus and Escherichia coli having moderate MIC at 402.4 μg/mL and 567.5 μg/mL. The findings support the traditional claims associated with A. chinense and indicate its potential as a source of bioactive compounds with antioxidant and antimicrobial properties, thereby justifying its relevance in traditional systems of medicine.