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Imaging LexA degradation in cells explains regulatory mechanisms and heterogeneity of the SOS response
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ABSTRACT
The SOS response functions as the central regulator of DNA repair and mutagenesis in most bacteria and stands as a paradigm of gene networks controlled by a master transcriptional regulator, LexA. We developed a single-molecule imaging approach to directly monitor the LexA repressor inside live
Escherichia coli
cells, demonstrating key mechanisms by which DNA-binding and degradation of LexA regulates the SOS response
in vivo.
Our approach revealed that self-cleavage of LexA occurs frequently during unperturbed growth and causes substantial heterogeneity in LexA abundances across cells. LexA variability underlies SOS gene expression heterogeneity and triggers spontaneous SOS pulses, which enhance bacterial survival in anticipation of stress.
Title: Imaging LexA degradation in cells explains regulatory mechanisms and heterogeneity of the SOS response
Description:
ABSTRACT
The SOS response functions as the central regulator of DNA repair and mutagenesis in most bacteria and stands as a paradigm of gene networks controlled by a master transcriptional regulator, LexA.
We developed a single-molecule imaging approach to directly monitor the LexA repressor inside live
Escherichia coli
cells, demonstrating key mechanisms by which DNA-binding and degradation of LexA regulates the SOS response
in vivo.
Our approach revealed that self-cleavage of LexA occurs frequently during unperturbed growth and causes substantial heterogeneity in LexA abundances across cells.
LexA variability underlies SOS gene expression heterogeneity and triggers spontaneous SOS pulses, which enhance bacterial survival in anticipation of stress.
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lexA
Mutations in
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: Evidence for a Lethal
lexA
Null Phenotype Due to the Fels-2 Prophage
ABSTRACT
The LexA protein of
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Purified lexA protein is a repressor of the recA and lexA genes.
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Escherichia coli shows a pleiotropic response (the SOS response) to treatments that damage DNA or inhibit DNA replication. Previous evidence has suggested that the product of the l...
Control of the LexA regulon by pH: evidence for a reversible inactivation of the LexA repressor during the growth cycle of Escherichia coli
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