Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Phenotypes of lexA Mutations in Salmonella enterica : Evidence for a Lethal lexA Null Phenotype Due to the Fels-2 Prophage
View through CrossRef
ABSTRACT
The LexA protein of
Escherichia coli
represses the damage-inducible SOS regulon, which includes genes for repair of DNA. Surprisingly,
lexA
null mutations in
Salmonella enterica
are lethal even with a
sulA
mutation, which corrects
lexA
lethality in
E. coli
. Nine suppressors of lethality isolated in a
sulA
mutant of
S. enterica
had lost the Fels-2 prophage, and seven of these (which grew better) had also lost the Gifsy-1 and Gifsy-2 prophages. All three phage genomes included a homologue of the
tum
gene of coliphage 186, which encodes a LexA-repressed cI antirepressor. The
tum
homologue of Fels-2 was responsible for
lexA
lethality and had a LexA-repressed promoter. This basis of
lexA
lethality was unexpected because the four prophages of
S. enterica
LT2 are not strongly UV inducible and do not sensitize strains to UV killing. In
S. enterica
,
lexA
(Ind
−
) mutants have the same phenotypes as their
E. coli
counterparts. Although
lexA
null mutants express their error-prone DinB polymerase constitutively, they are not mutators in either
S. enterica
or
E. coli
.
Title: Phenotypes of
lexA
Mutations in
Salmonella enterica
: Evidence for a Lethal
lexA
Null Phenotype Due to the Fels-2 Prophage
Description:
ABSTRACT
The LexA protein of
Escherichia coli
represses the damage-inducible SOS regulon, which includes genes for repair of DNA.
Surprisingly,
lexA
null mutations in
Salmonella enterica
are lethal even with a
sulA
mutation, which corrects
lexA
lethality in
E.
coli
.
Nine suppressors of lethality isolated in a
sulA
mutant of
S.
enterica
had lost the Fels-2 prophage, and seven of these (which grew better) had also lost the Gifsy-1 and Gifsy-2 prophages.
All three phage genomes included a homologue of the
tum
gene of coliphage 186, which encodes a LexA-repressed cI antirepressor.
The
tum
homologue of Fels-2 was responsible for
lexA
lethality and had a LexA-repressed promoter.
This basis of
lexA
lethality was unexpected because the four prophages of
S.
enterica
LT2 are not strongly UV inducible and do not sensitize strains to UV killing.
In
S.
enterica
,
lexA
(Ind
−
) mutants have the same phenotypes as their
E.
coli
counterparts.
Although
lexA
null mutants express their error-prone DinB polymerase constitutively, they are not mutators in either
S.
enterica
or
E.
coli
.
Related Results
Impact of the duffy-null phenotype on hydroxyurea dosing and clinical outcomes in children with sickle cell disease
Impact of the duffy-null phenotype on hydroxyurea dosing and clinical outcomes in children with sickle cell disease
Abstract
Background: Individuals with Duffy-null phenotype have lower circulating neutrophils without an increased risk ...
Lower neutrophil counts after hematopoietic stem cell transplant for sickle cell disease patients using donors with duffy-null phenotype
Lower neutrophil counts after hematopoietic stem cell transplant for sickle cell disease patients using donors with duffy-null phenotype
Abstract
Background: Individuals with the Duffy null phenotype (red blood cells that lack the Fya and Fyb antigens) have...
Prophage acquisition by
Staphylococcus aureus
contributes to the expansion of Staphylococcal immune evasion
Prophage acquisition by
Staphylococcus aureus
contributes to the expansion of Staphylococcal immune evasion
Abstract
Staphylococcus aureus
colonizes 30% of the human population, but only a few clones cause severe infections.
...
Canine Cystitis Caused by Salmonella enterica subsp. enterica
Canine Cystitis Caused by Salmonella enterica subsp. enterica
Background: Urinary tract infection in dogs is usually associated with the presence of bacteria, with a higher prevalence of Gram-negative bacteria, represented mainly by enteric b...
Potable Water Sources, Household Hygiene, and Sanitation Practices in Ikpoba Okha LGA, Edo State: Implications for Public Health and Sustainable Water Management
Omoregie, Andrew Edosa.1 Omoregie Abieyuwa Peace2 Okoro, Enyinnaya Okoro.3
1 College of Medi
Potable Water Sources, Household Hygiene, and Sanitation Practices in Ikpoba Okha LGA, Edo State: Implications for Public Health and Sustainable Water Management
Omoregie, Andrew Edosa.1 Omoregie Abieyuwa Peace2 Okoro, Enyinnaya Okoro.3
1 College of Medi
BACKGROUND
Access to potable drinking water and sufficient sanitation continues to be an urgent global concern, particularly in developing regions where con...
Gamma (γ)-radiation stress response of the cyanobacterium
Anabaena
sp. PCC7120: Regulatory role of LexA and photophysiological changes
Gamma (γ)-radiation stress response of the cyanobacterium
Anabaena
sp. PCC7120: Regulatory role of LexA and photophysiological changes
Abstract
High radioresistance of the cyanobacterium,
Anabaena
sp. PCC7120 has been attributed to efficient DN...
Laboratory Epidemiology of
Salmonella
Infections and Multi-Drug Resistance Profiles in Nigeria: Barriers, Challenges and Proposed Solutions
Laboratory Epidemiology of
Salmonella
Infections and Multi-Drug Resistance Profiles in Nigeria: Barriers, Challenges and Proposed Solutions
Abstract
Background
National surveillance data on
Salmonella
antim...
Prevalence and Antimicrobial Susceptibility of Salmonella enterica in Milk supply chain, Humans, and milking environment in Woliata Sodo, Ethiopia
Prevalence and Antimicrobial Susceptibility of Salmonella enterica in Milk supply chain, Humans, and milking environment in Woliata Sodo, Ethiopia
Abstract
Background: Foodborne disease caused by Salmonella enterica is among the leading causes of death worldwide. Few studies have been conducted on the prevalence of Sa...