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Gamma (γ)-radiation stress response of the cyanobacterium Anabaena sp. PCC7120: Regulatory role of LexA and photophysiological changes
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Abstract
High radioresistance of the cyanobacterium,
Anabaena
sp. PCC7120 has been attributed to efficient DNA repair, protein recycling, and oxidative stress management. However, the regulatory network involved in these batteries of responses remains unexplored. In the present study, the role of a global regulator, LexA in modulating gamma (γ)-radiation stress response of
Anabaena
was investigated. Comparison of the cytosolic proteome profiles upon γ-radiation in recombinant
Anabaena
strains, AnpAM (vector-control) and An
lexA
+
(LexA-overexpressing), revealed 41 differentially accumulated proteins, corresponding to 29 distinct proteins. LexA was found to be involved in the regulation of 27 of the corresponding genes based on the presence of AnLexA-Box, EMSA, and/or qRT-PCR studies. The majority of the regulated genes were found to be involved in C-assimilation either through photosynthesis or C-catabolism and oxidative stress alleviation. Photosynthesis, measured in terms of PSII photophysiological parameters and thylakoid membrane proteome was found to be affected by γ-radiation in both AnpAM and An
lexA
+
cells, with LexA affecting them even under control growth conditions. Thus, LexA functioned as one of the transcriptional regulators involved in modulating γ-radiation stress response in
Anabaena
. This study could pave the way for a deeper understanding of the regulation of γ-radiation-responsive genes in cyanobacteria at large.
Highlights
γ-radiation alters PSII photophysiology and thylakoid proteome profile in
Anabaena
.
LexA modulates the cytosolic and thylakoid proteome of
Anabaena
under γ-radiation.
LexA functions as one of the regulators of radiation-responsive genes in
Anabaena
.
Title: Gamma (γ)-radiation stress response of the cyanobacterium
Anabaena
sp. PCC7120: Regulatory role of LexA and photophysiological changes
Description:
Abstract
High radioresistance of the cyanobacterium,
Anabaena
sp.
PCC7120 has been attributed to efficient DNA repair, protein recycling, and oxidative stress management.
However, the regulatory network involved in these batteries of responses remains unexplored.
In the present study, the role of a global regulator, LexA in modulating gamma (γ)-radiation stress response of
Anabaena
was investigated.
Comparison of the cytosolic proteome profiles upon γ-radiation in recombinant
Anabaena
strains, AnpAM (vector-control) and An
lexA
+
(LexA-overexpressing), revealed 41 differentially accumulated proteins, corresponding to 29 distinct proteins.
LexA was found to be involved in the regulation of 27 of the corresponding genes based on the presence of AnLexA-Box, EMSA, and/or qRT-PCR studies.
The majority of the regulated genes were found to be involved in C-assimilation either through photosynthesis or C-catabolism and oxidative stress alleviation.
Photosynthesis, measured in terms of PSII photophysiological parameters and thylakoid membrane proteome was found to be affected by γ-radiation in both AnpAM and An
lexA
+
cells, with LexA affecting them even under control growth conditions.
Thus, LexA functioned as one of the transcriptional regulators involved in modulating γ-radiation stress response in
Anabaena
.
This study could pave the way for a deeper understanding of the regulation of γ-radiation-responsive genes in cyanobacteria at large.
Highlights
γ-radiation alters PSII photophysiology and thylakoid proteome profile in
Anabaena
.
LexA modulates the cytosolic and thylakoid proteome of
Anabaena
under γ-radiation.
LexA functions as one of the regulators of radiation-responsive genes in
Anabaena
.
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