Identifikasi cDNA Gen RB pada Tanaman Kentang Produk Rekayasa Genetika Katahdin SP951

<p>To overcome the negative impact of the fungicide application, RB gene, originated from Solanum bulbocastanum, a resistance<br />gene to the disease has been introduced to the genome of potato plant Katahdin, which was later named as Katahdin SP951.<br />The aims of the research were to confirm the RB gene in the genetically modified plant (GMP) Katahdin SP951 through<br />resequencing the RB gene in the Katahdin SP951 genome and to align the RB genes isolated from the transgenic Katahdin SP951<br />and the cloning vector pLCD0454, which was used the vector to transform Katahdin cultivar. The RB gene has high similarity to<br />other resistance genes in potato plants, and therefore nontransgenic Katahdin, and Solanum bulbocastanum were used in the<br />analysis as the negative and positive control, respectively. Seven pairs of specific primers, which could differentiate the RB gene<br />were used for gene sequencing studies. The sequencing experiments obtained 2913 bases which showed 100% similarity to the<br />RB sequence isolated from the pLCD04541 plasmid. It is concluded that the RB gene in the Katahdin SP951 did not experience<br />mutation during transformation process and integration into the Katahdin genome.</p>