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A simple and practical agglutination assay for human leucocyte antigen‐B27 typing
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Background and Objectives The human leucocyte antigen (HLA) B27 is the most frequently typed single antigen that is associated with diseases. Here, we describe a simple and rapid particle agglutination assay (PaGIA) for HLA‐B27 typing.Materials and Methods Superparamagnetic particles were coated with a monoclonal antibody to HLA‐B27 and subsequently used for testing. Anticoagulated whole‐blood samples were obtained from healthy blood donors (n = 194) with known HLA patterns and from patients (n = 51) who had been typed positive for HLA‐B27 by flow cytometry.Results The particles agglutinated only after incubation with HLA‐27‐positive blood samples, using the ID‐microtyping system. Positive reactions were clearly distinguishable from negative reactions in all samples tested. Flow cytometric HLA‐B27 typing revealed an indeterminate result in one patient.Conclusions The new HLA‐B27 PaGIA is suitable for rapid typing of HLA‐B27. The assay is simple and easy to perform, and can be implemented in any routine laboratory.
Title: A simple and practical agglutination assay for human leucocyte antigen‐B27 typing
Description:
Background and Objectives The human leucocyte antigen (HLA) B27 is the most frequently typed single antigen that is associated with diseases.
Here, we describe a simple and rapid particle agglutination assay (PaGIA) for HLA‐B27 typing.
Materials and Methods Superparamagnetic particles were coated with a monoclonal antibody to HLA‐B27 and subsequently used for testing.
Anticoagulated whole‐blood samples were obtained from healthy blood donors (n = 194) with known HLA patterns and from patients (n = 51) who had been typed positive for HLA‐B27 by flow cytometry.
Results The particles agglutinated only after incubation with HLA‐27‐positive blood samples, using the ID‐microtyping system.
Positive reactions were clearly distinguishable from negative reactions in all samples tested.
Flow cytometric HLA‐B27 typing revealed an indeterminate result in one patient.
Conclusions The new HLA‐B27 PaGIA is suitable for rapid typing of HLA‐B27.
The assay is simple and easy to perform, and can be implemented in any routine laboratory.
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