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Immunoradiometric assay for the rapid detection of HLA‐B27
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SummaryA new method for the rapid detection of the HLA‐B27 antigen is discussed which consists of a direct immunoradiometric assay (IRA) utilizing a 125I‐labelled, HLA‐B27 specific monoclonal antibody to detect HLA‐B27 in whole citrated blood. Thus far, the assay has been used to assign HLA‐B27 status in a population study involving 142 individuals; 36 subjects were HLA‐B27+ by conventional microcytotoxicity and all 36 were detected by the new IRA and all 106 B27‐ individuals were also accurately assigned by IRA. The problem of detection of the cross‐reactive HLA antigen HLA‐B7 was overcome by blocking the reactive sites on HLA‐B7 molecules with the HLA‐B7 specific antibody, BB71. This new assay enables accurate detection of HLA‐B27 within 30 min of venepuncture and should facilitate the assessment of HLA‐B27 status in tissue typing laboratories.
Title: Immunoradiometric assay for the rapid detection of HLA‐B27
Description:
SummaryA new method for the rapid detection of the HLA‐B27 antigen is discussed which consists of a direct immunoradiometric assay (IRA) utilizing a 125I‐labelled, HLA‐B27 specific monoclonal antibody to detect HLA‐B27 in whole citrated blood.
Thus far, the assay has been used to assign HLA‐B27 status in a population study involving 142 individuals; 36 subjects were HLA‐B27+ by conventional microcytotoxicity and all 36 were detected by the new IRA and all 106 B27‐ individuals were also accurately assigned by IRA.
The problem of detection of the cross‐reactive HLA antigen HLA‐B7 was overcome by blocking the reactive sites on HLA‐B7 molecules with the HLA‐B7 specific antibody, BB71.
This new assay enables accurate detection of HLA‐B27 within 30 min of venepuncture and should facilitate the assessment of HLA‐B27 status in tissue typing laboratories.
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