Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Immunoradiometric assay for the rapid detection of HLA‐B27
View through CrossRef
SummaryA new method for the rapid detection of the HLA‐B27 antigen is discussed which consists of a direct immunoradiometric assay (IRA) utilizing a 125I‐labelled, HLA‐B27 specific monoclonal antibody to detect HLA‐B27 in whole citrated blood. Thus far, the assay has been used to assign HLA‐B27 status in a population study involving 142 individuals; 36 subjects were HLA‐B27+ by conventional microcytotoxicity and all 36 were detected by the new IRA and all 106 B27‐ individuals were also accurately assigned by IRA. The problem of detection of the cross‐reactive HLA antigen HLA‐B7 was overcome by blocking the reactive sites on HLA‐B7 molecules with the HLA‐B7 specific antibody, BB71. This new assay enables accurate detection of HLA‐B27 within 30 min of venepuncture and should facilitate the assessment of HLA‐B27 status in tissue typing laboratories.
Title: Immunoradiometric assay for the rapid detection of HLA‐B27
Description:
SummaryA new method for the rapid detection of the HLA‐B27 antigen is discussed which consists of a direct immunoradiometric assay (IRA) utilizing a 125I‐labelled, HLA‐B27 specific monoclonal antibody to detect HLA‐B27 in whole citrated blood.
Thus far, the assay has been used to assign HLA‐B27 status in a population study involving 142 individuals; 36 subjects were HLA‐B27+ by conventional microcytotoxicity and all 36 were detected by the new IRA and all 106 B27‐ individuals were also accurately assigned by IRA.
The problem of detection of the cross‐reactive HLA antigen HLA‐B7 was overcome by blocking the reactive sites on HLA‐B7 molecules with the HLA‐B7 specific antibody, BB71.
This new assay enables accurate detection of HLA‐B27 within 30 min of venepuncture and should facilitate the assessment of HLA‐B27 status in tissue typing laboratories.
Related Results
Coexistence of Behçet's Disease and Ankylosing Spondylitis
Coexistence of Behçet's Disease and Ankylosing Spondylitis
Whether Behçet's disease (BD) is of the seronegative spondyloarthropathy (SSpA) group has been a subject of debate for many years.[] Behçet's syndrome is now not considered to belo...
High Resolution HLA-A, HLA-B, and HLA-C Allele Frequencies in Romanian Hematopoietic Stem Cell Donors
High Resolution HLA-A, HLA-B, and HLA-C Allele Frequencies in Romanian Hematopoietic Stem Cell Donors
The HLA genes are associated with various autoimmune pathologies, with the control of the immune response also being significant in organs and cells transplantation. The aim of the...
Diversity of HLA Class I and II Genes in the North Indian Population
Diversity of HLA Class I and II Genes in the North Indian Population
Introduction: Numerous studies have concentrated on specific populations to explore the extensive polymorphism of class I and II HLA genes. This genetic diversity is crucial for va...
A simple and practical agglutination assay for human leucocyte antigen‐B27 typing
A simple and practical agglutination assay for human leucocyte antigen‐B27 typing
Background and Objectives The human leucocyte antigen (HLA) B27 is the most frequently typed single antigen that is associated with diseases. Here, we describe a simple and rapid ...
KIR-HLA Signal System Genetic Background of Donor and Recipient Determines Outcomes in HLA-Identical Sibling HSCT.
KIR-HLA Signal System Genetic Background of Donor and Recipient Determines Outcomes in HLA-Identical Sibling HSCT.
Abstract
Objective: To explore the relationship between the background of donor -recipient KIR-HLA and the outcomes in HLA-identical sibling HSCT in Chinese.
...
Unveiling the Link: HLA-B27 Genetic Marker and Interleukin Levels in Psoriatic Arthritis Patients
Unveiling the Link: HLA-B27 Genetic Marker and Interleukin Levels in Psoriatic Arthritis Patients
This study aimed to examine the occurrence of Human leukocyte antigen-B27 (HLA-B27) and its subtypes, alongside the levels of the inflammatory markers Interleukin-17 and Interleuki...
Diagnostic accuracy of PD weighed MRI /STIR sequence to determine periarticular bone marrow edema of <10mm depth in predicting acute sacroilliatis in relation with HLA B27
Diagnostic accuracy of PD weighed MRI /STIR sequence to determine periarticular bone marrow edema of <10mm depth in predicting acute sacroilliatis in relation with HLA B27
Background: Acute sacroiliitis is a critical early feature of axial spondyloarthritis (axSpA), and timely diagnosis is essential to prevent irreversible structural damage. Magnetic...
Diagnostic accuracy of PD weighed MRI /STIR sequence to determine periarticular bone marrow edema of <10mm depth in predicting acute sacroilliatis in relation with HLA B27
Diagnostic accuracy of PD weighed MRI /STIR sequence to determine periarticular bone marrow edema of <10mm depth in predicting acute sacroilliatis in relation with HLA B27
Background: Acute sacroiliitis is a critical early feature of axial spondyloarthritis (axSpA), and timely diagnosis is essential to prevent irreversible structural damage. Magnetic...