Potensi antimikroba minyak atsiri daun jeruk (Citrus)

Background: Some plants that are included in the genus Citrus are one of the plants producing essential oils which is a natural substance that is known to have an antibacterial effect.Objectives: The study aims to determine the ability of essential oils from the leaves of several types of citrus (Citrus) in inhibiting the growth of Escherichia coli.Methods: An experimental study using a complete randomized design consisting of 4 treatments and four replications each. The treatments consisted of distilled water (control), lime leaf essential oil, kaffir lime essential oil and lime leaf essential oil. Microbiology test uses agar diffusion method.Results: Anova test showed that essential oils from some lime can inhibit the growth of Escherichia coli (p = 0.000). Duncan's further test results showed that the average diameter of the inhibition zone formed showed that the average diameter of the largest inhibition zone was formed in the treatment which was given lime orange essential oil (16.55 mm) and was not significantly different (p <0.05) with orange essential oil kaffir (15.40 mm) and lime essential oil (14.97 mm). Based on the Duncan test results showed that there is no difference in the average diameter of inhibitory zones between essential oils of lime, lime and lime, this can be caused because the three lime are classified in the same genus, namely the genus citrus.Conclusion: Essential oils from some lime can inhibit the growth of Escherichia coli. The average diameter of the inhibition zone that formed showed the greatest inhibition formed in the treatment which was given lime essential oil.