RESPONS PERTUMBUHAN KALUS Catharanthus roseus YANG DIBERI PERLAKUAN TRIPTOFAN

This research aims are to find out the induction and growth respons of C. roseus callus on the tryptophan treatments. Three steps have been done in this research, 1) callus induction, 2) culture callus that treated tryptophan, 3) qualitative and quantitative assay callus culture.  The concentration of tryptophan (ppm) that treated are 0 (T0), 100 (T1), 125 (T2), 150 (T3), 175 (T4), 200 (T5) and 225  (T6). The research results showed that the MS media for callus induction was NAA 2 ppm and Kinetin 0,2 ppm that produced compact callus with hairy roots. Fresh and dry weight of C. roseus callus that treated with tryptophan decreased  at 100, 125, 150 ppm and 225 ppm. However, callus that treated 175 and 200 ppm tryptophan inccreased. The highest growth of callus occurred at callus 200 ppm (T4) with the dry weight 0,251 g. The tryptophan affected of enzymes induction and other activities for sinthethized of alkaloid.  These enzymes correlated with decreased of the callus growth. The callus growth that treated tryptophanm better than callus control  after 40th day subculture. The callus control growth showed necrotic and brownees.