Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
BETA2/NeuroD Protein Can Be Transduced Into Cells Due to an Arginine- and Lysine-Rich Sequence
View through CrossRef
BETA2/NeuroD, a basic helix-loop-helix transcription factor, is a key regulator of pancreatic islet morphogenesis and insulin gene transcription. Here we report for the first time that the BETA2/NeuroD protein can permeate several cells, including pancreatic islets, due to an arginine- and lysine-rich protein transduction domain sequence in its structure. The BETA2/NeuroD protein was transduced in a dose-dependent manner up to 1 μmol/l. Transduced BETA2/NeuroD functions similarly to endogenous BETA2/NeuroD: it binds to the insulin promoter and activates its expression. We also investigated the mechanism of BETA2/NeuroD protein transduction. The BETA2/NeuroD protein penetrated cells by macropinocytosis and was released from endosomes homogeneously in cytoplasm and nuclei. These data suggest that BETA2/NeuroD protein transduction could be a safe and valuable strategy for enhancing insulin gene transcription without requiring gene transfer technology.
American Diabetes Association
Title: BETA2/NeuroD Protein Can Be Transduced Into Cells Due to an Arginine- and Lysine-Rich Sequence
Description:
BETA2/NeuroD, a basic helix-loop-helix transcription factor, is a key regulator of pancreatic islet morphogenesis and insulin gene transcription.
Here we report for the first time that the BETA2/NeuroD protein can permeate several cells, including pancreatic islets, due to an arginine- and lysine-rich protein transduction domain sequence in its structure.
The BETA2/NeuroD protein was transduced in a dose-dependent manner up to 1 μmol/l.
Transduced BETA2/NeuroD functions similarly to endogenous BETA2/NeuroD: it binds to the insulin promoter and activates its expression.
We also investigated the mechanism of BETA2/NeuroD protein transduction.
The BETA2/NeuroD protein penetrated cells by macropinocytosis and was released from endosomes homogeneously in cytoplasm and nuclei.
These data suggest that BETA2/NeuroD protein transduction could be a safe and valuable strategy for enhancing insulin gene transcription without requiring gene transfer technology.
Related Results
7
th
International Symposium on Enabling Technologies for Life Sciences (ETP)
7
th
International Symposium on Enabling Technologies for Life Sciences (ETP)
The seventh in the series of ETP Symposia (see
Rapid Communications in Mass Spectrometry
2012,
26
, ...
TINGKAT PROTEIN DAN LISIN DALAM RANSUM TERHADAP EFISIENSI LISIN DAN PROTEIN NETTO PADA AYAM KAMPUNG UMUR 12 MINGGU
TINGKAT PROTEIN DAN LISIN DALAM RANSUM TERHADAP EFISIENSI LISIN DAN PROTEIN NETTO PADA AYAM KAMPUNG UMUR 12 MINGGU
Penelitian yang dilakukan ini dalam mencari pengaruh tingkat protein dan lisin terhadap efisiensi lisin dan penggunaan protein netto pada ayam kampung yang diperlihara sampai umur ...
Effect of l-arginine and l-lysine supplementation in low-protein feeds on the growth of native chickens in the starter phase
Effect of l-arginine and l-lysine supplementation in low-protein feeds on the growth of native chickens in the starter phase
Some feeds that are used as protein sources, such as fish meal and soybean meal, have a relatively high cost. Therefore, synthetic amino acids, such as arginine and lysine, can be ...
Leishmania
parasite arginine deprivation response pathway influences the host macrophage lysosomal arginine sensing machinery
Leishmania
parasite arginine deprivation response pathway influences the host macrophage lysosomal arginine sensing machinery
Abstract
Extensive interaction between the host and pathogen metabolic networks decidedly shapes the outcome of infection. Infection with
...
Sustained Long Term Engraftment and Transgene Expression of Peripheral Blood CD34+ Cells Transduced with Advanced Lentiviral Vectors in the Preence of ABC Inhibitors.
Sustained Long Term Engraftment and Transgene Expression of Peripheral Blood CD34+ Cells Transduced with Advanced Lentiviral Vectors in the Preence of ABC Inhibitors.
Abstract
Because mobilized peripheral blood (MPB) represents an attractive source of cells for gene therapy, we investigated the ability of advanced-generation lenti...
Microencapsulated Escape Lysine with Tannin as an Adjuvant in Sheep Diets
Microencapsulated Escape Lysine with Tannin as an Adjuvant in Sheep Diets
The use of escape protein, which is absorbed in the small intestine, can improve the production of ruminant animals because it meets their protein requirements better. This study h...
Co-Relation between Plasma Beta2 Microglobulin Level and Cardiac Performance Factors in CKD Patients
Co-Relation between Plasma Beta2 Microglobulin Level and Cardiac Performance Factors in CKD Patients
Background: Cardiovascular disease mortality and morbidity is astonishingly high in CKD population. As renal function decreases beta2 microglobulin level starts to rise. There is a...
Modeling the response of Japanese quail to arginine intake
Modeling the response of Japanese quail to arginine intake
Background
Understanding how Japanese quails respond to arginine intake has been an objective that previous studies have not fulfilled. The main responses to be quan...