The histological assessment of Anabolic Androgenic Steroids (Nandrolone Decanoate) administration on brain and liver of male albino rats

Nandrolone Decanoate is an anabolic androgenic steroid as testosterone derivatives which was commonly used in several diseases anemia, osteoporosis and muscle atrophy as well as its use by bodybuilders. The aim of this study to assessment the role of Nandrolone Decanoate administration on histological architecture of brain and liver and serum SOD, GSH Adropin in albino rats. Fifteen male albino rats, random distributed into three groups, 1st group I, 2nd group II where subcutaneous injection with (0. 5 and 0.15) ml/kg/ day / four times a week, for 45 day respectively and control group were injected with normal saline. The result of The group I showed congestion of meningeal and cortex hemolyzed blood vessels, cortex foamy appearance, neurons degeneration of inner pyramidal layer, pyramidal cells atrophy. axonal atrophy, myelin sheath degeneration and glial cells degeneration. The results of Group II, revealed infiltration of inflammatory cells within meningeal membrane, showed pyramidal cells degeneration within outer layer of cortex, atrophy of neuron cells and apoptosis of glial cells of inner cortex. Foamy appearance of white matter. The results of liver histological examinations of group I showed aggregate of fibroblast and inflammatory cells around central vein, infiltration of inflammatory cells in sinusoids vessels in between hepatocytes, cytoplasmic vacuolation of hepatocytes, pyknosis of degenerative hepatocytes, pyknosis of hepatocytes, hemorrhage in between hepatocytes with hemosiderin deposition and necrosis. The results of group II showed vacuolation within engorged hemolyzed RBCs within central vein, Infiltration of fibroblast cells adjacent portal bile duct, massive necrosis of hepatocytes. The results of Prussian blue staining in liver samples of group I and II showed iron deposit adjacent central vein, and between hepatocytes and increased iron accumulation between hepatocytes related with dosage of given dose. On the other hand the results of the present study revealed significant increasing of malondialdehyde and Adropin serum level otherwise there is a significant decreased superoxide dismutase and Glutathione when compared with control group on level P≤ 0.05.