Evaluation of the quality of native and cryopreserved semen of birds of breeds brama light and brama fawn and their chimeras

Due to the significant decline in the diversity of bird breeds and populations, there is a need to preserve germ cells of rare breeds and unique organisms, such as chimeras. The main method for preserving the gene pool in birds is the method of semen cryopreservation, which is used in many countries of the world in the form of creating cryobanks. Sperm from the birds of the breeds Brama light and Brama fawn and their chimeras were obtained by abdominal massage. Cryopreservation of the semen was carried out in small granules with the addition of a 6% solution of dimethylacetamide. The granules were thawed on a heated metal plate at 60°C. The ejaculate volume, sperm activity and its concentration in the studied breeds and their chimeras did not differ statistically. The maximum activity of the frozen-thawed semen was observed in the semen of the Brahma fawn breed (40.0%), and the minimum activity in the Brahma light breed was 30%, while in chimeras the semen activity occupied an intermediate position and amounted to 35%. Thus, frozen-thawed spermatozoa of bird chimeras are viable, as well as native semen.