Aktivitas Antibakteri Sabun Antijerawat Ekstrak Daun Sirih (Piper betle L.)

A study has been conducted on the antibacterial activity of transparent acne soap containing betel leaf extract (Piper betle L.). This study aims to determine the antibacterial activity and irritation potential of transparent soap containing betel leaf extract (Piper betle L.). Betel leaf extract was obtained by maceration using ethanol solvent and delipidated using a liquid-liquid method with n-hexane and ethanol solvents. Betel leaf extract was formulated into transparent soap preparations with concentrations of 2%, 4%, and 6% using melting method. The transparent soap was tested for its activity against Staphylococcus epidermidis ATCC 25923 and Propionibacterium acnes ATCC 26442, which cause acne, using the liquid dilution and solid dilution methods. The transparent soap was tested for its irritation potential using the open patch method on the skin behind the ears of volunteers. The results of the antibacterial activity test showed a Minimum Bactericidal Concentration (MBC) of 2%. The irritation test results showed that all formulas did not cause irritation. It was concluded that ethanol extract of betel leaf (Piper betle L.) can be formulated into a transparent soap preparation that has activity at a concentration of 2% and is safe for topical use