Development of HPLC Based QuEChERS Method for High Recovery of Fungicides From Vegetable Samples

Abstract Vegetables are the most essential part of our diet. Several pesticides are used to kill the pests. These pest cause different diseases on vegetable plants. As a result of which they are getting more and more production of vegetables but there are many hazards for humans. To get rid of this problem, a simple QuEChERS method is used which is based on solvent extraction method in which different salts and acetonitrile were used. In this method clean-up process and extraction were important HPLC technique is used to determine the accurate results. Despicable recoveries of fungicides were ranges between 0.63% to 107.68% at spiking levels and at different factors through percentage RSD between 3.6% to 4.7%. LOD of two fungicides were in the range of 0.0021mg/kg to 0.00181mg/kg and LOQ of two fungicides were in the range of 0.0706mg/kg to 0.00606mg/kg. By varying many factors the recoveries of fungicides may vary. The recoveries of two fungicides may increase by increasing temperature, pH and concentration but decrease with the passage of time due to degradation of fungicides. By this QuEChERS method we get different concentration of fungicides in some vegetables but in Methi vegetables they are absent. And determinations and extraction of fungicides were completed by HPLC technique. QuEChERS method is very efficient and good method for determination of pesticides in various vegetables. Hence our experimental results were validated with this efficient method.