Evaluation of Phytochemical Constituents and Antibacterial Activity of Chromolaena Odorata L. Leaf Extract against Selected Multidrug Resistant Bacteria Isolated from Wounds

Aim: To study the antibacterial effect of Chromolaena odorata extracts on multidrug resistant Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae from wounds. Place of Study: University of Medical Sciences Teaching Hospital, Akure, Ondo State, Nigeria, between January and June 2019. Methodology: A total of 87 wound swabs were collected from patients of University of Medical Sciences Teaching Hospital, Akure. Air-dried and powdered C. odorata leaves were extracted using hot water, ethanol and methanol as extraction solvents and concentrated using a rotary evaporator. The concentrated C. odorata extracts were purified using chromatographic techniques. Qualitative and quantitative phytochemical screening of C. odorata extracts were done by standard methods. Antibiotics susceptibility pattern of bacterial isolates to a panel of ten (10) conventional antibiotics was determined by disc diffusion method. Results: C. odorata methanolic extract had the highest extract yield (26.2%). From the multidrug resistance analysis, 66.7% of bacterial isolates tested had multidrug resistance index (MDRI) of 100%. Pseudomonas aeruginosa was susceptible to 100 mg/ml of C. odorata ethanolic extract but resistant (0.00±0.00) to 100 mg/ml of hot aqueous C. odorata extract. Conclusion: This study reveals the inhibitory activities of C. odorata extracts on multidrug resistant bacteria isolated from wounds and an indication of their potential in the treatment of wound infection.