Antimicrobial activity of Loranthus europaeus L. and Lawsonia inermis L. extracts against clinical Methicillin-resistant Staphylococcus aureus isolated from boil infections

This study included to collect 106 samples boil swab from patients, males and females, at Baqubah Teaching Hospital whose ages ranged between (1-80) years old. At the period of 1st July 2016 to the end of January 2017. Aimed to identification of bacterial isolates and determine the antibiotic resistance of isolates. In addition to determine the activity of aqueous and alcohol of Loranthus europaeus and Lawsonia inermis extracts on bacterial isolates. The results showed a significant difference (p <0.05) in the percentage of infected females (58.49%) compared to the infected males (41.51%). The study involved isolation and diagnosis of (41) (38.67%) of Staphylococcus aureus, of which, 5 (12.20%) were methicillin-resistant isolates. Bacterial isolates were identified initially depending on cultural and microscopic features and biochemical reactions test. Antibiotics sensitivity of Staph. aureus conducted for 14 β-lactam antibiotic groups. All of bacterial isolates (100%) were resistant to ceftriaxone, cefotaxime and cefepime, and  (92.68%) to cefoxitin and penicillin G, while (100%) of isolates were sensitive to Imipenem and Meropenem. Regarding plant extracts, various concentrations of hot aqueous extracts were prepared for each one of them (12.5, 25, 50, 100 and 200) mg/ml against methicillin-resistant Staph. aureus. When Loranthus europaeus’s extract was used, the diameters of  inhibition zone were (14.57, 15.28,16.00, 23.71 and 25.00 mm) while (0.00, 11.85, 18.00, 21.28 and 24.14 mm) when Lawsonia inermis’s extract was used. Like aqueous extracts, same range of concentrations were prepared for alcoholic extracts. The diameters of  inhibition zone were (7.57, 8.42, 10.57,13.28, 17.28), (11.14, 17.00, 20.85, 25.42, 28.42) when alcoholic extracts of Loranthus europaeus and  Lawsonia inermis, respectively were used. Comparing the activity of hot and alcoholic aqueous extracts of the tested plants, alcoholic extracts of Lawsonia inermis had a higher inhibitory effect against S. aureus isolates.