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Prevalence of Community Acquired Methicillin Resistant Staphylococcus Aureus (Ca-Mrsa) In The Nasal Cavity of Delta State University Students.
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INTRODUCTION Staphylococcus aureus (SA) is
a Gram positive, opportunistic bacterium that
frequently colonizes the oral cavity, nasal cavity,
and skin of the healthy people. This can cause a
variety of localized and invasive problems ranging
from superficial skin infections to life threatening
Pneumonia and bloodstream infections
OBJECTIVE: The study is aimed at investigating
the prevalence of community acquired methicillin
resistant Staphylococcus aureus in the nasal cavity
of Delta State University students
METHOD:100 nasal swabs(samples) were
collected from the anterior nares of both male and
female students of DELSU using Sterile swab
sticks and was cultured on mannitol salt Agar
.After culturing on mannitol salt Agar, all the
Staphylococcus aureus specie were
isolated.Standard identification test was carried out
by performing Gram staining test and other
biochemical test like coagulase test, Catalase test
and Fermentation test were further used to
determine the identity of the organism. Antibiotic
susceptibility test was carried out for
Staphylococcus aureus using the Agar well
diffusion method.The zone of inhibition in
millimeters was measured with a meter rule. Theaverage readings were taken to be the zone of
inhibition and were compared with KirbyBauer
Standard for Staphylococcus aureus. zones of
inhibitions within the range(≤10)were used to
determine the Methicillin resistant Staphylococcus
aureus(MRSA).
RESULT: Out of the 100 samples collected and
cultured on the mannitol salt agar, 93 were staph
species why 33(35.5%) were found to be
Staphylococcus aureus why the remaining
60(64.5%) were other specie of Staphylococcus
aureus .Only the staph aureus specie were worked
on and out of the 33(35.5%) Staphylococcus
aureus, 23(69.7%) of the Staphylococcus aureus
were found to be methicillin resistant why
10(30.3%) were found to be sensitive to Oxacillin
disk which was used as a reference to methicillin
antibiotics because its closely related to methicillin.
CONCLUSION: The result from this study shows
increased Staphylococcus aureusresistance to
methicillin(Oxacillin),therefore community
acquired methicillin resistant Staphylococcus
aureus is prevalent in the nasal cavity of DELSU
Students and therefore suggest that more work has
to be done as an individual and even in the clinical
setting and in the community to curtail the spread
of methicillin resistant Staphylococcus aureus.
Title: Prevalence of Community Acquired Methicillin Resistant Staphylococcus Aureus (Ca-Mrsa) In The Nasal Cavity of Delta State University Students.
Description:
INTRODUCTION Staphylococcus aureus (SA) is
a Gram positive, opportunistic bacterium that
frequently colonizes the oral cavity, nasal cavity,
and skin of the healthy people.
This can cause a
variety of localized and invasive problems ranging
from superficial skin infections to life threatening
Pneumonia and bloodstream infections
OBJECTIVE: The study is aimed at investigating
the prevalence of community acquired methicillin
resistant Staphylococcus aureus in the nasal cavity
of Delta State University students
METHOD:100 nasal swabs(samples) were
collected from the anterior nares of both male and
female students of DELSU using Sterile swab
sticks and was cultured on mannitol salt Agar
.
After culturing on mannitol salt Agar, all the
Staphylococcus aureus specie were
isolated.
Standard identification test was carried out
by performing Gram staining test and other
biochemical test like coagulase test, Catalase test
and Fermentation test were further used to
determine the identity of the organism.
Antibiotic
susceptibility test was carried out for
Staphylococcus aureus using the Agar well
diffusion method.
The zone of inhibition in
millimeters was measured with a meter rule.
Theaverage readings were taken to be the zone of
inhibition and were compared with KirbyBauer
Standard for Staphylococcus aureus.
zones of
inhibitions within the range(≤10)were used to
determine the Methicillin resistant Staphylococcus
aureus(MRSA).
RESULT: Out of the 100 samples collected and
cultured on the mannitol salt agar, 93 were staph
species why 33(35.
5%) were found to be
Staphylococcus aureus why the remaining
60(64.
5%) were other specie of Staphylococcus
aureus .
Only the staph aureus specie were worked
on and out of the 33(35.
5%) Staphylococcus
aureus, 23(69.
7%) of the Staphylococcus aureus
were found to be methicillin resistant why
10(30.
3%) were found to be sensitive to Oxacillin
disk which was used as a reference to methicillin
antibiotics because its closely related to methicillin.
CONCLUSION: The result from this study shows
increased Staphylococcus aureusresistance to
methicillin(Oxacillin),therefore community
acquired methicillin resistant Staphylococcus
aureus is prevalent in the nasal cavity of DELSU
Students and therefore suggest that more work has
to be done as an individual and even in the clinical
setting and in the community to curtail the spread
of methicillin resistant Staphylococcus aureus.
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