Callus induction treatments influence antimicrobial effect of tissue culture-derived Thuja orientalis L.

Thuja orientalis belonging to the family Cupresseaceae is an important forest tree species for timber and wood production. Its wood is light in weight and highly resistant to decay. The leaf of explants of this plant was cultured for callus induction and plant regeneration. The explants of this plant were cultured onto Murashig and Skoog (MS) medium supplemented with different concentrations of (α- naphtalene acetic acid (NAA), pchlorophenoxyisobutyric acid (PCIB) alone and in combination with 6- benzyladenine (BA) for callus induction. The highest callus was induced in medium containing 3 mg/l PCIB. The lowest callus was found to be on MS medium containing (3 mg/l NAA +0.1 mg/l BA). Callus of explants were grown on MS media contained 3 mg/l PCIB +0.1 mg/l BA extract is the most active fraction against E. coli also it is active against Streptococcus pneumonia and Bcilis subtilis. Callus of explants were grown on MS media (3 mg/l NAA +0.1 mg/l BA) has higher activity against Streptococcus pneumonia, Callus of explants were grown on MS media supplemented with (3 mg/l PCIB +0.1 mg/l BA) has higher activity against Bacilis subtilis. These results provide a basis for future studies on genetic improvement and could be applied to production of secondary metabolites through cell culture in Thuja orientalis.