An Exploratory Clinical Study of Organic Go Ghrita Administered Orally and Through Pratimarsha Nasya in Children with Autism Spectrum Disorder with Digital Nadi Assessment

Background: AutismSpectrumDisorder (ASD) is a complex neurodevelopmental condition characterized by restricted, repetitive behaviours and deficits in social communication. Conventional therapies have limited efficacy in addressing core symptoms, prompting exploration of integrative approaches. In Ayurveda, Go Ghrita (cow ghee) is described as a Medhya Rasayana with potential neuropsychological benefits. Methods: A single-arm, open- label exploratory clinical study was conducted on 30 children aged 3–16 years diagnosed with ASD according to INCLEN criteria, supplemented with Nadi assessment. Organic Go Ghrita prepared from Tharparkar breed cows, fed on five desert grasses (Sewan, Dhaman, Ganthia, Bekar, Bhunt), was administered orally in age-appropriate doses along with Pratimarsha Nasya. The primary outcome was the change in ISAA scores, while secondary outcomes included the Parental Stress Index, behavioural observations, and Nadi Tarangini parameters. Results: There was a statistically significant reduction in ISAA scores from baseline (100.63 ± 12.54) to post-treatment (83.17 ± 10.21), representing a 17.39% improvement (Z = -4.78, p < 0.001). The Parental Stress Index showed a significant decrease of 22.16% (p < 0.001). Digital Nadi assessment revealed significant Vata pacification (49.59%) with simultaneous restoration of Pitta and Kapha parameters (p < 0.001). No adverse events were reported during the study. Discussion: The Medhya Rasayana, and lipophilic properties of Organic Go Ghrita may influence neurocognitive processes by modulating the gut–brain axis, neuroinflammation, and autonomic homeostasis. Digital Nadi assessment offers objective evaluation of Doshic and autonomic patternsin ASD,whilePratimarshaNasya potentiallyfacilitates direct drug delivery to the brain via olfactory and trigeminal pathways. Conclusion: This exploratory study provides preliminary evidence supporting the safety and potential therapeutic role of Organic Go Ghrita, administered orally and via Nasya, in children with ASD. Additionally, it highlights the utility of digital Nadi Tarangini assessment in integrative pediatric research.