Production of secondary metabolites in Streptomyces and their impact in biotechnology: A Systematic Review Protocol v1

Actinobacteria are recognized for their ability to produce diverse secondary metabolites with significant pharmaceutical and industrial value. Streptomyces is traditionally viewed as the most prolific genus, yet recent genomic studies suggest that other Actinobacteria may possess important but underexplored biosynthetic capacities. This systematic review aims to compare the production, diversity, and applications of secondary metabolites from Streptomyces and other Actinobacteria. The review will search PubMed and Scopus for experimental, observational, and computational studies published within the last ten years. Selected articles will be screened by two independent reviewers, and relevant data (such as metabolite types, biosynthetic gene clusters, yields, biological activities, and biotechnological applications) will be extracted into a standardized table. The methodological quality of each study will also be evaluated. The collected data will be synthesized qualitatively, and quantitative comparisons will be considered when sufficient comparable information is available. The results will provide an updated overview of the biosynthetic potential of Actinobacteria and help identify genera with promising value for future biotechnological development.