Molecular, morphological, and biochemical identification of sembada merah and sembada hitam rice (Oryza sativa L)

Abstract Sembada Merah and Sembada Hitam rice were Sleman Yogyakarta Local Genetic Resources which have potential as a functional food. This study aimed to determine the molecular, morphological, and biochemical character of Sembada Hitam and Sembada Merah rice. Molecular identification using RM 220, RM 224 and RM 252 microsatellite markers was carried out in Genetics and Breeding Laboratory of Agriculture Faculty of UGM Yogyakarta. Morphological identification including plant, stem, leaf, flower, panicle and grain character was carried out at AIAT Yogyakarta, meanwhile biochemical identification (amylose and anthocyanin (cyanidin-3-glucoside) content) was conducted in Indonesian Center for Rice Research. Those two rice varieties were identified with Segreng Handayani as a check. The results showed that Sembada Hitam and Sembada Merah rice differ of molecularly, morphologically, and biochemically so that Sembada Hitam and Sembada Merah rice are separate varieties.