Dyskeratotic Process of Hyperkeratosis lenticularis perstans (Flegel)

The dyskeratotic changes in the lesions of hyperkeratosis lenticularis perstans were examined electron microscopically and histochemically. Under electron microscopy, the formation of keratohyalin granules was reduced and there was a lack of lamellar granule formation. In addition, the catabolic processes were disturbed, and ribosomal and mitochondrial structures remained, and lipid crystal-like structures were seen in the stratum corneum. The structure of the desmosomes of the stratum corneum was identical to that seen in the stratum spinosum. Staining with thiol-specific, fluorescent reagent, N-(7-dimethylamino-4-methyl-coumarinyl) maleimide (DACM) demonstrated strong fluorescence in the cytoplasm of the stratum corneum and granulosum without reduction by 2-mercaptoethanol. With reduction, moderate fluorescence was only seen at the periphery of the stratum corneum cells. These results indicate that there is a cystine-rich protein in the stratum corneum cells.