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Janelia Atalanta series plasmid cloning v1
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The Janelia Atalanta plasmids allows simple cloning of a gRNA and homology arms for CRISPR/Cas9 mediated homology directed repair in Drosophila. Gateway-compatible arms are synthesized with appropriate attL recognition sequences and homology arms required for homology directed repair. One, or two, of the arms also encodes a sgRNA flanked by tRNA sequences, which is expressed at high levels from a U6 promoter. The sgRNA is spliced out of the transcript by endogenous tRNA maturation cellular machinery.
Title: Janelia Atalanta series plasmid cloning v1
Description:
The Janelia Atalanta plasmids allows simple cloning of a gRNA and homology arms for CRISPR/Cas9 mediated homology directed repair in Drosophila.
Gateway-compatible arms are synthesized with appropriate attL recognition sequences and homology arms required for homology directed repair.
One, or two, of the arms also encodes a sgRNA flanked by tRNA sequences, which is expressed at high levels from a U6 promoter.
The sgRNA is spliced out of the transcript by endogenous tRNA maturation cellular machinery.
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