Determination of the Appropriate Application Rate of Inoculant for Enhanced Soybean Production

Abstract Soybean productivity in sub-Saharan Africa is often constrained by low soil fertility and limited biological nitrogen fixation (BNF). This study evaluated the effects of different rhizobial inoculant rates (0, 5, 7.5, 10, and 12.5 g/kg seed) on growth, yield, and Biological Nitrogen Fixation (BNF) of three soybean varieties, Afayak, Favour, and Jenguma, using a split-plot design with variety as the main plot and inoculant rate as the subplot. Significant effects of variety and inoculant rate were observed for key agronomic traits. Compared to the uninoculated control, grain yield increased (p < 0.001) in 2023 by 27.3%, 78.8%, 45.9%, and 146.5%, and in 2024 by 47.6%, 54.2%, 87.9%, and 180.6% at 5, 7.5, 10, and 12.5 g/kg seed, respectively. The 10 g/kg rate resulted in the highest nitrogen fixation: 51.5 kg/ha in 2023 and 56.4 kg/ha in 2024. Jenguma responded best to inoculation, followed by Afayak and Favour. Economic analysis using partial budgeting and marginal rate of return (MRR) identified the most profitable rates as those exceeding a 100% MRR threshold. Results underscore the importance of variety-specific inoculation strategies to enhance nitrogen use efficiency and sustainable soybean production in the region.