Comparative Assessment of Lupeol in Wild plant and In Vitro Callus Culture of Hemidesmus indicus by HPLC Technique

Medicinal plants are used as traditional medicines throughout the world for thousands of years and continue to provide new remedies to mankind. Plants are one of the richest sources of secondary metabolites. The roots of Hemidesmus indicus served as the remedy for leprosy, syphilis, leucoderma, asthma, dysentery, fever and blood, kidney and urinary diseases. The aim of the present study was to determine the quantity of lupeol present in callus and wild plant extract of Hemidesmus indicus. The root callus was initiated on MS medium fortified with varied concentrations and combination of different auxins like, 2-4 Dichloro acetic acids (2-4D), napthalene acetic acid (NAA) and cytokinin like, kinetin (Kn)  in 250 ml culture flasks. The content of lupeol in callus and root extracts was analyzed by HPLC technique. The highest percentage of callus induction (88.00 %) was observed in MS medium (C7) containing 1.0 mg/l NAA with highest callus growth in terms of fresh weight (946.48±6.6) which resulted in soft friable callus. The callus produced by C7 was used selected for further study. The HPLC study revealed that the quantity of lupeol present in callus extract was more compared to the wild plant extract of Hemidesmus indicus.