Development of Chromatographic Profile of Processed and non-processed Piper nigrum Linn.

Advanced Chromatographic technique is one of the parameters for qualitative and quantitative assessment. In this study advanced chromatographic studies were conducted for the assessment of processed and non-processed Piper nigrum Linn.  Ayurvedic pharmaceutical is enriched in a wide range of preparations. The specific methods are applied for different formulation preparations.  Laghumaini vasant is a herbomineral formulation in which one of the ingredients is P. nigrum. In this formulation, the special process is advocated for P.nigrum.Linn. P nigrum should be soaked in buttermilk till the outer black covering is removed. There are possibilities that some changes may occur due to this process. Hence this study focused on the comparative assessment of processed P. nigrum and non-processed P.nigrum. For assessment chromatography techniques were used. HPLC and HPTLC methods were used to get more precise results.   High-Performance Thin Layer and High performance Liquid Chromatography were studied by using piperine as a reference standard. The piperine bands were seen in both processed and non-processed P.nigrum when analysed through both techniques. But, HPLC method has shown that piperine recovery is more in processed P.nigrum. In non-processed P.nigrum twenty seven peaks were observed but after processing with Buttermilk twenty one peaks were seen. The small six peaks were missing in processed P.nigrum.