SPECTROSCOPIC ANALYSIS OF CHOLESTEROL ENZYME CHOX IN PVA ON THE INDICATOR ELECTRODE MEMBRANE OF CHOLESTEROL ANALYTE WITH THE POTENTIOMETRIC BIOSENSOR METHOD

The spectrum pattern analysis of the urease enzyme immobilization technique in PVA, a PVA polymer that is soluble in warm water, has been carried out. There are differences in the spectrum patterns of PVA, PVA-KTpClPB and PVAEnzyme-KTpClPB solutions, respectively UVA, UVB and UVC. The purpose of this study was to create a cholesterol sensor whose analyte cholesterol on the indicator electrode was coated with a PVA-Enzyme cholesterol (ChOx)/GA 2.9%/PANI-p-toluenesulfonic acid/PVC-KTpClPB-o-NPOE 61% membrane. The method used is the potentiometric biosensor method of ChOx enzyme immobilization technique in PVA, which is symbolized by (PVA-E ChOx). This study was conducted by comparing the indicator electrode membrane coated with PVA- variation of Enzyme cholesterol (ChOx)/GA 2.9%/PANI-benzenesulfonic acid/PVC-KTpClPB-o-NPOE 61%. ChOx enzyme was varied at 1:1 and 1:3. The results of the UV-Vis analysis of ChOx enzyme immobilization in PVA obtained a spectrum pattern that was the same as the ChOx spectrum pattern on the ChOx enzyme 1:1. After obtaining the best results from the PVA-E ChOx membrane at 1:1. Continued coating of the first indicator electrode GA 2.9% / PANI-ptoluenesulfonic acid / PVC-KTpClPB-o-NPOE 61%. The second electrode GA 2.9% / PANI-benzenesulfonic acid / PVC-KTpClPB-o-NPOE 61%. Both membranes were analyzed by EDX, XRD, and FTIR, each placed on a tungsten wire and glass preparation, to determine the best membrane. The best membrane is PVA-E1 ChOx / GA 2.9% / PANIp-toluenesulfonic acid / PVC-KTpClPB-o-NPOE 61%.