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Reactivation of latent pseudorabies virus infection in vaccinated commercial sows

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SUMMARY Pseudorabies virus (prv) was isolated from 9 of 44 prv-vaccinated seropositive sows on 5 of 11 farms. Although serum-neutralization antibody titers were 1:16 to 1:256, 28 virus isolates were obtained from tonsil, nasal, or buccal swab samples from 9 sows given 2 ml of dexamethasone/kg of body weight im for 5 days. Pseudorabies virus was isolated from 6 of 20 sows (3 of 5 farms) given a killed-virus vaccination. Virus was obtained from 3 of 24 sows (2 of 6 farms) given modified-live virus and killed-virus vaccination. Evaluation of the 9 prv with 5 restriction endonucleases revealed 4 prv existing genotypes. The 9 isolated types of prv appeared to be indistinguishable by Kpn I and BamHI restriction endonuclease analysis; however, when analyzed with Sal I, HinfI, and Pst I, isolates 7 (farm D), 8 (farm C), and 9 (farm B) had numerous differences. Isolates 1, 2, 3, and 4 (farm F) and 5 and 6 (farm G) appeared to be the same genotype when further analyzed with Pst I, HinfI, and Sal I.
Title: Reactivation of latent pseudorabies virus infection in vaccinated commercial sows
Description:
SUMMARY Pseudorabies virus (prv) was isolated from 9 of 44 prv-vaccinated seropositive sows on 5 of 11 farms.
Although serum-neutralization antibody titers were 1:16 to 1:256, 28 virus isolates were obtained from tonsil, nasal, or buccal swab samples from 9 sows given 2 ml of dexamethasone/kg of body weight im for 5 days.
Pseudorabies virus was isolated from 6 of 20 sows (3 of 5 farms) given a killed-virus vaccination.
Virus was obtained from 3 of 24 sows (2 of 6 farms) given modified-live virus and killed-virus vaccination.
Evaluation of the 9 prv with 5 restriction endonucleases revealed 4 prv existing genotypes.
The 9 isolated types of prv appeared to be indistinguishable by Kpn I and BamHI restriction endonuclease analysis; however, when analyzed with Sal I, HinfI, and Pst I, isolates 7 (farm D), 8 (farm C), and 9 (farm B) had numerous differences.
Isolates 1, 2, 3, and 4 (farm F) and 5 and 6 (farm G) appeared to be the same genotype when further analyzed with Pst I, HinfI, and Sal I.

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