HPTLC FINGER PRINT PROFILE OF METHANOLIC EXTRACT OF HALYMINIYA DILATATA AND LIAGORA CERNOIDES AS THERAPEUTIC AGENTS

Analysis of pharmaceutical and natural compounds is commonly used in all stage of drug discovery and development process. High – performance thin layer chromatography is one of the sophisticated instrumental techniques based on the full capabilities of thin layer chromatography. The present study is focused to develop the high performance thin layer chromatography (HPTLC) fingerprint profile of methanolic extracts of marine red alga H.dilatata and L. ceranoides. The aim of present study is to perform the qualitative phytochemical analysis of red seaweeds H.dilatata and L.ceranoides. Among the 2 seaweeds, H.dilatata and L.cernoides showed the of active constituents in the ethanol, methanol and aqueous extracts. H.dilatata and L. ceranoides showed of compounds are present in three extracts. HPTLC profiling of the algal extract confirm about the presence of various phytochemicals. HPTLC method is used for the separation of the active constituents and TLC of these extracts on silica gel pre-coated aluminum plates of Merck by the automatic TLC applicator and using the solvent system Chloroform : Methanol (6.0 : 4.0) from H.dilatata and another solvent system like Ethyl acetate : Formic acid (7.3: 2.7 : 0.01) from L.ceranoides was performed. The HPTLC finger print scanned at 254 and 366 nm for method for extraction 12 µl was performed algal extract revealed in the seaweeds of H.dilatata 5 peaks and L.ceranoides shows the 10 peaks and with Rf value in the range of both seaweeds 68.38 to 2.10 and 45.80 to 0.53 respectively.