Chromatographic Analysis of Polyherbal Extract and Formulation by HPTLC and GC-MS Methods

Background: The World Health Organization (WHO) acknowledges that natural plants and their bioactive components have been recognized for their potential therapeutic benefits. The main issue facing the herbal industry is the lack of effective quality control for herbal extracts and formulations Establishment standards are essential to ensuring the efficacy and integrity of natural medicine development. Determining the precise compound responsible for therapeutic action poses an equally challenging task, as these compounds frequently work synergistically to produce medicinal benefits. Aim: In this exploration, GC-MS and high-performance thin-layer chromatography were used to analyze the formulation and polyherb extract. The HPTLC technique is often employed to analyze the sample and compare its fingerprint to reference standards to verify plant extracts’ quality and formulations’ quality. Using GC-MS, the phytochemical ingredients in the formulation and the extract have been determined. Methods: This research assessed a mixture of herb extract and formulation using analytical approaches. The combined hydroalcoholic extracts of the herbs were used to produce the polyherb extract of seeds of Azadirachta indica, Carica papaya, Trigonella foenum-graecum, Gossipium herbaceum, fruit of Piper nigrum. The required excipients were added before compressing the herbal mixture extract into tablets. Results: The extract and formulation’s HPTLC chromatograms displayed Rf values of 0.14, 0.34, 0.48, 0.63, and 0.76.The results of the GC-MS, N-Hexadecanoic, 1,19-Eicosadiene, 9,12-Octadecadienoic Acid, Z, Z-4,16-Octadecadien-1-Ol Acetate, Piperine that were analyzed provide different peaks to determining the presence of phytochemicals found in the extract and formulation were confirmed. Conclusion: HPTLC and GC-MS aid in the identification of individual chemical constituents within herbal formulations by coupling chromatographic and mass spectral data of the sample with reference standards or databases, the identity of key compounds can be confirmed, ensuring authenticity, and has shown an intense correlation between the mixture of herb extract and formulation. Major Findings: In this Research, GC-MS and high-performance thin-layer chromatography were used to analyze the formulation and polyherbal extract of key compounds can be confirmed, ensuring authenticity, and has shown an intense correlation between the mixture of herb extract and formulation.