Detection of Mn – Dependent Chitinase for Wheat Root Rot Disease Control by Real time PCR

The use of some mineral salts with concentrations of 10, 30 and 50 mm Muller led to the inhibition ofthe activity of the enzyme chitinase, but to varying degrees. Manganese chloride MnCl2 showed moreeffect in stimulating the activity of the enzyme, as the effectiveness reached 74.87% in addition to calciumchloride CaCl2, which had a stimulating effect for the enzyme as well. The salts EDTA and NH4Cl had aninhibitory effect on the enzyme activity. The field experiment was conducted for the purpose of inducingsystemic resistance of wheat plants with the fungus T. longibrachiatum under conditions of infection withthe pathogen Fusarium oxysporum and evaluating the efficacy of the chitinase enzyme in improving plantgrowth for three varieties of wheat, which are Iba 99, Sham 6 and July 2 for 45 days, after which thevegetative characteristics were taken. For cultivated plants and the efficacy of the chitinase enzyme for theshoot and root system, the induction treatment (pathogenic fungus + T. longibrachiatum + MnCl2) proved itsrole in the highest reduction of the severity of infection with the pathogen fungus of wheat, reaching 18.05%and the high vegetative and root growth indicators under conditions of pathogen infection and for all thestudied varieties. The results of induction were due to the increased expression of the chitinase enzyme gene.RT - PCR technique was used. It showed that all the studied cultivars with 99, Sham 6 and July 2 had higherexpression than the control treatment.