The Crocus Sativus Stigma Extract as Anticancer for SKOV-3 Cell Line

Cancer is an uncontrolled growth and division of abnormal cells with the capacity to spread to other body regions (metastasis). The aim of this study is to investigate the effect of saffron aqueous extract an anticancer on the ovarian cancer the material and method included preparation the saffron aqueous extract and SKOV-3 after maintained of cell cultures in RPMI-1640 supplemented with 10% Fetal bovine serum, penicillin and streptomycin. Next, two methods were used to investigate the impact of saffron. One of these methods involves measuring the impact of saffron on the mitochondrial activity of SKOV-3 cells using the fluorescent dye Rhodamine (Rh123). Furthermore, the activation statuses of p53 and caspase-3 were evaluated using a fluorescein caspase-3 and p53 staining kit. Eventually, the results were promising because the saffron extract caused damage in mitochondria and a significant increase by caspase-3 and p53 that caused to apoptosis SKOV-3 cells without effect on normal cells. Finally, the usage of saffron supplements in addition to established treatment procedures may benefit people with ovarian cancer since saffron extract triggers cancer cell apoptosis through several indications that manifest differently.