Pelomonas baiyunensis sp. nov., Pelomonas candidula sp. nov., Pelomonas dachongensis sp. nov., Pelomonas lactea sp. nov., Pelomonas margarita sp. nov., Pelomonas nitida sp. nov., Pelomonas parva sp. nov. and Roseateles rivi sp. nov., isolated from streams

Eight Gram-stain-negative, aerobic and motile strains (BYS78WT, BYS87WT, BYS96WT, BYS180WT, DC23WT, DXS20WT, LKC17WT and LYH14WT) were isolated from streams in China and showed the highest 16S rRNA gene sequence similarities with the species of genus Pelomonas. The calculated average nucleotide identity, digital DNA–DNA hybridization and average amino acid sequence identity values among these eight strains and other closely related strains were less than 89.8, 39.1 and 89.4 %, respectively, indicating that each of the eight strains should represent an independent novel species. The further reconstructed phylogenomic tree showed that seven isolated strains clustered closely with the Pelomonas strains. Only strain BYS180WT clustered with the Roseateles strains. The phylogenomic tree also showed that the taxonomic status of the recently published species (Paucibacter sediminis, Roseateles aquae, ‘Roseateles caseinilyticus’, ‘Roseateles cellulosilyticus’, Roseateles subflavus and Roseateles violae) was questionable. ‘R. caseinilyticus’ and ‘R. cellulosilyticus’ should be reassigned to the genus Pelomonas as Pelomonas caseinilytica P7T (=KACC 22504T=TBRC 15694T) and Pelomonas cellulosilytica P8T (=KACC 22505T=TBRC 15695T), respectively. P. sediminis, R. aquae, R. subflavus and R. violae should be reassigned to the genus Kinneretia as Kinneretia sediminis, Kinneretia aquae, Kinneretia subflava and Kinneretia violae, respectively. Combining the above descriptions, strains BYS78WT, BYS87WT, BYS96WT, DC23WT, DXS20WT, LKC17WT and LYH14WT should represent seven novel species of the genus Pelomonas, for which the names Pelomonas baiyunensis sp. nov. (type strain BYS87WT=GDMCC 1.4090T=KCTC 8151T), Pelomonas candidula sp. nov. (type strain BYS78WT=GDMCC 1.4089T=KCTC 8152T), Pelomonas dachongensis sp. nov. (type strain DC23WT=GDMCC 1.4087T=KCTC 8148T), Pelomonas lactea sp. nov. (type strain DXS20WT=GDMCC 1.4088T=KCTC 8147T), Pelomonas margarita sp. nov. (type strain LKC17WT=GDMCC 1.4086T=KCTC 8146T), Pelomonas nitida sp. nov. (type strain BYS96WT=GDMCC 1.4091T=KCTC 8150T) and Pelomonas parva sp. nov. (type strain LYH14WT=GDMCC 1.4276T=KCTC 8301T) are proposed, respectively. Strain BYS180WT should represent a novel species of the genus Roseateles, for which the name Roseateles rivi sp. nov. (type strain BYS180WT=GDMCC 1.4098T=KCTC 8149T) is proposed.