Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
FlyTomo: A streamlined software for on-the-fly cryo-ET data processing and diagnosis
View through CrossRef
Abstract
Cryo-electron tomography (Cryo-ET) combined with subtomogram averaging (STA) enables the structural elucidation of macromolecular assemblies in their native environments. However, the widespread adoption of cryo-ET has been limited by its labor-intensive, expertise-dependent data processing workflow. Here we present FlyTomo, a software that streamlines data processing from frame alignment to STA with high-throughput for authentic cryo-ET scenarios. During data acquisition, FlyTomo performs real-time diagnosis, enabling prompt feedback on sample quality, microscope performance and structural features. After acquisition, it aggregates diagnostic metrics into an interactive overview, guiding users through data review and refinement. FlyTomo also curates raw and processed data into structured directories to simplify data management and archiving. We validated FlyTomo across a diverse set of authentic cryo-ET samples, including purified enveloped viruses and cryo-lamellae, on multiple microscopes and cameras, achieving structures at resolutions ranging from 3.4 to 5.7 Å. Collectively, by integrating accuracy, scalability and usability, FlyTomo reduces the technical barrier for in situ structural biology using cryo-ET.
Cold Spring Harbor Laboratory
Title: FlyTomo: A streamlined software for on-the-fly cryo-ET data processing and diagnosis
Description:
Abstract
Cryo-electron tomography (Cryo-ET) combined with subtomogram averaging (STA) enables the structural elucidation of macromolecular assemblies in their native environments.
However, the widespread adoption of cryo-ET has been limited by its labor-intensive, expertise-dependent data processing workflow.
Here we present FlyTomo, a software that streamlines data processing from frame alignment to STA with high-throughput for authentic cryo-ET scenarios.
During data acquisition, FlyTomo performs real-time diagnosis, enabling prompt feedback on sample quality, microscope performance and structural features.
After acquisition, it aggregates diagnostic metrics into an interactive overview, guiding users through data review and refinement.
FlyTomo also curates raw and processed data into structured directories to simplify data management and archiving.
We validated FlyTomo across a diverse set of authentic cryo-ET samples, including purified enveloped viruses and cryo-lamellae, on multiple microscopes and cameras, achieving structures at resolutions ranging from 3.
4 to 5.
7 Å.
Collectively, by integrating accuracy, scalability and usability, FlyTomo reduces the technical barrier for in situ structural biology using cryo-ET.
Related Results
Bean seed fly (Delia platura, Delia florilega) and onion fly (Delia antiqua) incidence in England and an evaluation of chemical and biological control options
Bean seed fly (Delia platura, Delia florilega) and onion fly (Delia antiqua) incidence in England and an evaluation of chemical and biological control options
AbstractBean seed fly and onion fly are significant pests of alliaceous crops in the UK. Their activity was monitored using yellow water traps at three field sites in England in 20...
A comprehensive foundation model for cryo-EM image processing
A comprehensive foundation model for cryo-EM image processing
AbstractCryogenic electron microscopy (cryo-EM) has become a premier technique for high-resolution structural determination of biological macromolecules1–4. However, its widespread...
Exploring Large Language Models Integration in the Histopathologic Diagnosis of Skin Diseases: A Comparative Study
Exploring Large Language Models Integration in the Histopathologic Diagnosis of Skin Diseases: A Comparative Study
Abstract
Introduction
The exact manner in which large language models (LLMs) will be integrated into pathology is not yet fully comprehended. This study examines the accuracy, bene...
Awareness of Cryo Electro-Tomography among Dental Students
Awareness of Cryo Electro-Tomography among Dental Students
CryoElectronomography (CryoET) is indeed an imaging method used to create high resolution (~1-4 nm) three-dimensional viewpoints of specimen, usually physiological macromolecules a...
Streamlining Grant Applications - What are the probabilities a streamlined grant is fundable and that a fundable grant is streamlined?
Streamlining Grant Applications - What are the probabilities a streamlined grant is fundable and that a fundable grant is streamlined?
Background: Securing qualified peer reviewers for public granting agencies is challenging and to avoid needlessly overworking these volunteers, there is increasing reliance on tria...
Strengths of Geo Polymer Concrete by Adding Metakaoline
Strengths of Geo Polymer Concrete by Adding Metakaoline
Based0on the results obtained from this study0,the following Conclusions seems to be valid. The increase0in percentage replacement of Fly Ash with Metakaoline from 0% to 10.00% cau...
Study of the Design and Mechanical Properties of the Mix Proportion for Desulfurization Gypsum–Fly Ash Flowable Lightweight Soil
Study of the Design and Mechanical Properties of the Mix Proportion for Desulfurization Gypsum–Fly Ash Flowable Lightweight Soil
In order to solve the global problem of bridge head jumping caused by the insufficient compaction of the roadbed in the transition section of highways and bridges, a desulfurizatio...
Influence of the characteristic of input materials on formation and properties of sintered fly ash body
Influence of the characteristic of input materials on formation and properties of sintered fly ash body
Artificial aggregate from sintered fly ash is an example of material, which can be used solely on the basis of fly ash without any additions. However, to ensure optimal progress of...