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Immunoblotting v1
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This protocol describes collection of protein from cultured cells and immunoblotting, including immunoblotting of large proteins using a proprietary tris-acetate buffer system.
Title: Immunoblotting v1
Description:
This protocol describes collection of protein from cultured cells and immunoblotting, including immunoblotting of large proteins using a proprietary tris-acetate buffer system.
Related Results
Immunoblotting
Immunoblotting
Immunoblotting allows detection of a protein antigen immobilized on the protein-retaining membrane support such as nitrocellulose or polyvinylidene fluoride (PVDF). The detection o...
Development of an immunoblotting assay for serodiagnosis of Burkholderia mallei infection: the whole-cell proteome-based paradigm
Development of an immunoblotting assay for serodiagnosis of Burkholderia mallei infection: the whole-cell proteome-based paradigm
Background and Objectives: Burkholderia mallei is the leading cause of glanders, a highly transmittable and an OIE-no- tifiable disease of equidae. Despite the importance of B. mal...
Optimization, Expression, Purification, and Activity Identification of Anti-PD-1 scFv
Optimization, Expression, Purification, and Activity Identification of Anti-PD-1 scFv
Introduction:
Anti-PD-1 monoclonal antibodies have revolutionized cancer therapy but
face challenges, including immune-related adverse events, poor tumor penetr...
Preparing Protein Solutions for Immunoblotting
Preparing Protein Solutions for Immunoblotting
Most protein solutions can be mixed with sample buffer and analyzed directly by immunoblotting. Any protein extraction buffer or solvent that is compatible with gel electrophoresis...
MAF Protein Elicits Innate Resistance To Bortezomib In Multiple Myeloma
MAF Protein Elicits Innate Resistance To Bortezomib In Multiple Myeloma
Abstract
Multiple myeloma (MM) is a malignancy of terminally differentiated clonal plasma cells displaying significant molecular heterogeneity with 7 subgroups defin...
Immunoblotting v1
Immunoblotting v1
This protocol describes collection of protein from cultured cells and immunoblotting, including immunoblotting of large proteins using a proprietary tris-acetate buffer system....
Immunoblotting using precast gels v1
Immunoblotting using precast gels v1
Immunoblotting is a key technique to visualize changes in protein levels upon treatments. This technique can be challenging and established procedures are required to ensure reprod...
GW24-e3869 Cardiac Peroxisome Proliferator-Activated Receptor-y Expression is Modulated by Oxidative Stress in Acutely Infrasound-Exposed Rats
GW24-e3869 Cardiac Peroxisome Proliferator-Activated Receptor-y Expression is Modulated by Oxidative Stress in Acutely Infrasound-Exposed Rats
Objectives
Rats were exposed to infrasound for several weeks, and adverse effects on the cardiovascular system were demonstrated using morphology, histopathology,...