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Immunoblotting using precast gels v1
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Immunoblotting is a key technique to visualize changes in protein levels upon treatments. This technique can be challenging and established procedures are required to ensure reproducibility. Here we present our optimized protocol for immunoblotting of protein samples using precast gels and semiwet transfer. This protocol can be used to analyze samples from cell extracts and from in vitro reactions.
Title: Immunoblotting using precast gels v1
Description:
Immunoblotting is a key technique to visualize changes in protein levels upon treatments.
This technique can be challenging and established procedures are required to ensure reproducibility.
Here we present our optimized protocol for immunoblotting of protein samples using precast gels and semiwet transfer.
This protocol can be used to analyze samples from cell extracts and from in vitro reactions.
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