Hepatoprotective Activity of Isolated Flavonoids of Ficus glomerata in Carbon Tetrachloride Induced Hepatotoxicity in Rats

The present examination was planned to isolate flavonoids from Ficus glomerata leaves separates, and surveyed their hepatoprotective action against CCl4 instigated liver toxicity in rodents. The petroleum ether, ethanol and aqueous extracts were prepared and screened for phytochemical examination. The in vitro antioxidant activity namely DPPH, Superoxide Scavenging, total polyphenol content and total flavonol content activity of ethanol and aqueous extract were investigated. The distinctive portions were detached from ethanol extracts by utilizing column chromatography. The fractions F5, F7 and F8 were screened for hepatoprotective activity. The phytochemical study demonstrated the flavonoids and polyphenol components present in ethanol and aqueous extract. The findings of in viro antioxidant activity suggested the ethanol extract have higher quantity of polyphenol and flavonoid component compared to aqueous extract. The fractions F5, F7 and F8 shows prominent hepatoprotective activity by altogether diminishing the CCl4 initiated modification in SGOT, SGPT, ALP, ACP, total bilirubin and direct bilirubin in blood. The isolated compound altered the SOD, GPx, CAT and lipid peroxidation level, it indicates the antioxidant activity of isolated components. The findings of study recommend that the flavonoid compound F7 of Ficus glomerata showed higher hepatoprotective activity compared F5 and F8.