Cytomorphological Analysis of Liquid PRF Produced with DUO Fixed Angle Centrifuge (Process, France)

Background: Liquid PRF is a second-generation injectable platelet concentrate rich in platelets, leukocytes, and fibrinogen obtained by centrifugation of autologous blood. Methods: This study aims to analyze the cellular and Fibrinogen content of various types of Liquid PRF (C-PRF liquid, A-PRF liquid, i-PRF, Liquid Fibrinogen) obtained with the use of DUO Fixed Angle Centrifuge (PRF DUO, Process for PRF, Nice, France) with Vacumed FL tubes (code 44909) and/or original S-PRF Sticky tube. An average accumulation of thrombocytes of almost 1.5 times compared to whole blood was found. Due to the high concentration of platelets, PRF-Liquids contain important growth factors for tissue regeneration. Results: In this preliminary study, we have shown that the type of Liquid PRF with a higher content of Platelets (126.3% vs. 109.5%), Monocytes (127.6% vs. 84.6%), with a sufficient content of Lymphocytes (192.9% vs. 242.1%) and Neutrophilic Granulocytes (64.6% vs. 64.8%) and Fibrinogen (67.9% vs. 87.3%), is the i-PRF (700 rpm x 5') obtained with Vacumed FL tube (code 44909) with statistically insignificant differences compared to whole blood, while the content of Lymphocytes and Fibrinogen present in i-PRF (700 rpm x 5') obtained with PRF-S-Sticky tube is higher. In comparison, the content of cells and Fibrinogen obtained with the two methods of preparing PRP is much lower. Conclusions: This indicates that the liquid PRF is more suitable to be used in various cases of tissue regeneration such as facial aesthetics, intra-articular injection, peri-ulcerative injection, etc., is the i-PRF (700 rpm x 5') obtained with a Vacumed FL tube.