Flax Seed Oil Alleviates Caffeinated Energy Drink Induced Metamorphosis in Femur Weight and Relative Tissue Weight Index of Adult Male Albino Rat

Introduction: Consumption of caffeinated energy drinks has been increased worldwide specially in younger population in order to increase mental and physical performance. Side effects on various systems of human body have been reported due to their usage. Flax seed oil has been well known since ancient times owing to its beneficial effects and has now become a major part in our functional food. Aims & Objectives: To evaluate the Protective effects of flax seed oil on caffeinated energy drink induced changes in adult male albino rat femur weight and relative tissue weight index. Place and duration of study: This study was accomplished in department of Anatomy, at Shaikh Zayed Postgraduate Medical Institute Lahore for 8 weeks. Material & Methods: 32 adult male albino rats average weight (250-300g) were randomly divided into four groups of 8 animals each. Group A (Control) received corn oil 5ml/kg body weight by gavage in addition to basal diet daily for 8 weeks. Group B (Experimental) received caffeinated energy drink (15ml/kg body weight) and corn oil 5ml/kg body weight. Group C (Experimental) received caffeinated energy drink (15ml/kg body weight) and 40% of flax seed oil (5ml/kg body weight), while group D (Experimental) received caffeinated energy drink (15ml/kg body weight) and 60% flax seed oil (5ml/kg body weight) daily for 8 weeks respectively. The animals were weighed before and after experiment. After 8 weeks, they were dissected to remove both femur and each femur was weighed, further the right femurs were used for statistical purpose. Results: The mean difference in weight of femur was nonsignificant amongst experimental groups (p= 0.950). The relative tissue weight index (RTWI) was significant (p-value 0.001). Conclusion: Flax seed oil helped in restoring altered femur weight and RTWI changes caused by caffeinated energy drink in adult male albino rats.