Anti-hyperuricemic effect of Polydatin in model mice: promoting uric acid excretion and inhibiting XOD

Abstract Objective The aim of this study was to understand the role of anti-hyperuricemic mechanism and nephro-protective effects of polydatin. Methods The oxonate-induced hyperuricemia mice model was established and uric acids in serum were observed. Kidney tissues were used to detect gene contents of URAT1, OAT1 and OAT3 by real-time-PCR. and to detect pathological features .The activity of XOD in liver tissues of mice was detected . Results Polydatin significantly reduced serum urate levels in hyperuricemic mice. Polydatin significantly inhibited increasing tendency of the mRNA and the protein levels of OAT1 and OAT3 ,and decreasing tendency of the mRNA and the protein level of URAT1 in hyperuricemic mice. Polydatin significantly inhibited the level of XOD in liver tissues of mice in a concentration-dependent manner in hyperuricemic mice. Polydatin showed a protective effect on the pathological injury of kidney in hyperuricemic mice. Conclusion The anti-hyperuricemic effect of polydatin was related to the down-regulation of renal URAT1, up-regulated renal OAT1 and OAT3 and inhibition XOD in the hyperuricemic mice.