Optimization of HPLC Conditions for Analysis of Paracetamol in Jamu Pegal Linu in Pasar Besar Malang City Using Response Surface Methodology-Box-Behnken Design (RSM-BBD)

Based on the Indonesian Ministry of Health Regulation No.7 in 2012, herbal medicine is disallowed to contain synthetic chemicals or isolated products with medicinal properties. However, from the findings of BPOM, there are still many herbal medicine producers who add medicinal chemicals to herbal medicine, one of which is the addition of paracetamol in jamu pegal linu. This study aimed to obtain optimum HPLC conditions for the analysis of paracetamol in jamu pegal linu using the Response Surface Methodology-Box-Behnken Design, ensure the HPLC method has met the validation parameters, and determine the presence and levels of paracetamol contained in jamu pegal linu sold in Pasar Besar, Malang City. HPLC conditions that were optimized were the percentage of methanol mobile phase in water, flow rate, and column temperature. The HPLC condition optimization results were obtained at a percentage of methanol mobile phase in water of 34.4%, mobile phase flow rate of 1 mL/min, and column temperature of 30°C. Analysis of paracetamol content of paracetamol in jamu pegal using HPLC and method validation with test parameters including selectivity (λmax 245 nm; paracetamol retention time ± 2.6 minutes; resolution ± 2.5), linearity (r2 = 0.9984), LOD (2.44 ppm), LOQ (7.40 ppm), accuracy (97-102%), and precision (0.26-0.69%). The analysis of paracetamol content in the three samples of jamu pegal linu analyzed showed that negative for paracetamol and fulfilled the regulation of the Indonesian Ministry of Health Regulation No.7 in 2012.