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Figure 3 from A Small Molecule Reacts with the p53 Somatic Mutant Y220C to Rescue Wild-type Thermal Stability
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<p>Azaindole compounds stabilized p53 Y220C to WT levels. <b>A,</b> Chemical structures of the azaindole series. <b>B,</b> p53 Y220C-CL was fully labeled by the compounds and purified by size-exclusion chromatography, and its thermal stability was analyzed by differential scanning fluorimetry. The difference in Tm relative to unliganded p53 Y220C-CL is displayed. <b>C,</b> Crystal structure of p53 Y220C-CL bound to KG13 at 1.7 Å resolution. <b>D,</b> 1 μmol/L of p53 Y220C-CL or p53 Y220C-CL D228A was incubated with 10 μmol/L compound at 4°C for 24 hours. Adduct formation was analyzed by LC/MS.</p>
Title: Figure 3 from A Small Molecule Reacts with the p53 Somatic Mutant Y220C to Rescue Wild-type Thermal Stability
Description:
<p>Azaindole compounds stabilized p53 Y220C to WT levels.
<b>A,</b> Chemical structures of the azaindole series.
<b>B,</b> p53 Y220C-CL was fully labeled by the compounds and purified by size-exclusion chromatography, and its thermal stability was analyzed by differential scanning fluorimetry.
The difference in Tm relative to unliganded p53 Y220C-CL is displayed.
<b>C,</b> Crystal structure of p53 Y220C-CL bound to KG13 at 1.
7 Å resolution.
<b>D,</b> 1 μmol/L of p53 Y220C-CL or p53 Y220C-CL D228A was incubated with 10 μmol/L compound at 4°C for 24 hours.
Adduct formation was analyzed by LC/MS.
</p>.
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