Supplementary Figure 6 from A Small Molecule Reacts with the p53 Somatic Mutant Y220C to Rescue Wild-type Thermal Stability

<p>Supplementary Figure S6: Western blots and for 28°C IP and CETSA (A) H1299 cells stably expressing p53 Y220C or p53 R273C were transferred from 37°C to 28°C and p21/MDM2 levels were monitored by western blot at the indicated time points. (B) U2OS cells stably expressing p53 Y220C or parent were transferred to a 28°C incubator and p21/MDM2 levels were observed by western blot at the indicated time points. (C) Extracts from H1299 cells expressing p53 Y220C incubated at 37°C or 28°C for 24 hr were immunoprecipitated with the WT conformation antibody Pab1620. (D) CETSA western blot for p53 Y220C DMSO. (E) CETSA western blot for p53 Y220C treated with 100 μM KG13 for 1 hr. (F) CETSA western blot for p53 WT DMSO.</p>