Comparative Immunogenic Response of Live Vaccine of Lasota and Mukteswar Strains of Newcastle Disease Virus by Different Routes of Inoculation in Layer Chicken

Newcastle disease (ND) has been one of the great challenges to Poultry in Pakistan and dealt with vaccination. Mortality and morbidity that are associated with it are economically significant. Two vaccines produced against Newcastle disease virus are Live ND-LaSota and ND Mukteswar vaccines and their immunogenic effects in backyard poultry. For that purpose, a total 130 of 6-8 weeks old layer cockerels were inoculated with both vaccines by different routes including Ocular, drinking water and Injection and serum samples were collected at 14, 21 and 35 days’ post inoculation were analyzed by Heamaglutination inhibition test for antibody titers. The best route of administration for ND-LaSota was observed by ocular drops and ND- Mukteswar through injection. So it was concluded that both the vaccines needed booster doses in 4th to 5th week of primary inoculation. ND-LaSota vaccine was found to be best for primary induction.