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Affinity Tags for Protein Purification
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The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the
recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also
serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial
approach, carefully chosen affinity tags designed in tandem have proven to be very successful in
the purification of single proteins or multi-protein complexes. In this mini-review, the key features of
the most commonly used affinity tags are discussed. The affinity tags have been classified into two
significant categories, epitope tags, and protein/domain tags. The epitope tags are generally small peptides
with high affinity towards a chromatography resin. The protein/domain tags often perform double
duty as solubility enhancers as well as aid in affinity purification. Finally, protease-based affinity tag
removal strategies after purification are discussed.
Title: Affinity Tags for Protein Purification
Description:
The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the
recombinant proteins.
These tags help in protein purification.
Additionally, some affinity tags also
serve a dual purpose as solubility enhancers for challenging protein targets.
By applying a combinatorial
approach, carefully chosen affinity tags designed in tandem have proven to be very successful in
the purification of single proteins or multi-protein complexes.
In this mini-review, the key features of
the most commonly used affinity tags are discussed.
The affinity tags have been classified into two
significant categories, epitope tags, and protein/domain tags.
The epitope tags are generally small peptides
with high affinity towards a chromatography resin.
The protein/domain tags often perform double
duty as solubility enhancers as well as aid in affinity purification.
Finally, protease-based affinity tag
removal strategies after purification are discussed.
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