Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Probiotic lactobacilli mediate their immunoregulatory functions in intestinal cells via modulation of H3 histone acetylation
View through CrossRef
AbstractAimsProbiotics are known to maintain intestinal homeostasis through the regulation of the immune response of the host. Hence, the role of histone modifications as epigenetic agents on immune modulations by potential probiotic bacteria has been investigated.Methods and ResultsHuman colonic epithelial cells (Caco-2) pre-treated with class I histone deacetylase (HDAC) specific inhibitor, MS-275, were incubated either with potential probiotic bacteria (Limosilactobacillus fermentum MTCC 5898 and Lacticaseibacillus rhamnosus MTCC 5897) or Escherichia coli (ATCC 14948) as an inflammatory agent. Initially, transcriptional expression of potential immune-related genes (IL-6, IL-8, and hBD-2) was analyzed using RT-qPCR, and later H3 histone acetylation (H3Ac) at the promoter region of these genes was confirmed with a chromatin immunoprecipitation (ChIP) assay respectively. Potential probiotic L. fermentum (MTCC 5898) significantly suppressed (P < 0.05) the inhibitor-mediated elevated expression of immune-related genes while another strain L. rhamnosus (MTCC 5897), did not influence these gene expression results. In contrast, as an inflammatory agent, E. coli (ATCC 14948) synergistically augmented the expression of immune-related genes. Later, ChIP analysis confirmed the occurrence of H3 acetylation at these genes’ promoter regions, which was directly related to the transcriptional activity of host epithelial cells stimulated by L. fermentum and E. coli, respectively. But in the case of L. rhamnosus, MTCC 5897, acetylation did not follow the transcription pattern and potentiated H3Ac on the promoter regions of these genes.ConclusionsPotential probiotics used in the study were found to regulate the immune response of host cells through histone acetylation in a strain-specific manner.Significance and Impact of StudyOccurrence of probiotic-mediated regulation of immune genes by H3 acetylation in a strain-specific manner.
Oxford University Press (OUP)
Title: Probiotic lactobacilli mediate their immunoregulatory functions in intestinal cells via modulation of H3 histone acetylation
Description:
AbstractAimsProbiotics are known to maintain intestinal homeostasis through the regulation of the immune response of the host.
Hence, the role of histone modifications as epigenetic agents on immune modulations by potential probiotic bacteria has been investigated.
Methods and ResultsHuman colonic epithelial cells (Caco-2) pre-treated with class I histone deacetylase (HDAC) specific inhibitor, MS-275, were incubated either with potential probiotic bacteria (Limosilactobacillus fermentum MTCC 5898 and Lacticaseibacillus rhamnosus MTCC 5897) or Escherichia coli (ATCC 14948) as an inflammatory agent.
Initially, transcriptional expression of potential immune-related genes (IL-6, IL-8, and hBD-2) was analyzed using RT-qPCR, and later H3 histone acetylation (H3Ac) at the promoter region of these genes was confirmed with a chromatin immunoprecipitation (ChIP) assay respectively.
Potential probiotic L.
fermentum (MTCC 5898) significantly suppressed (P < 0.
05) the inhibitor-mediated elevated expression of immune-related genes while another strain L.
rhamnosus (MTCC 5897), did not influence these gene expression results.
In contrast, as an inflammatory agent, E.
coli (ATCC 14948) synergistically augmented the expression of immune-related genes.
Later, ChIP analysis confirmed the occurrence of H3 acetylation at these genes’ promoter regions, which was directly related to the transcriptional activity of host epithelial cells stimulated by L.
fermentum and E.
coli, respectively.
But in the case of L.
rhamnosus, MTCC 5897, acetylation did not follow the transcription pattern and potentiated H3Ac on the promoter regions of these genes.
ConclusionsPotential probiotics used in the study were found to regulate the immune response of host cells through histone acetylation in a strain-specific manner.
Significance and Impact of StudyOccurrence of probiotic-mediated regulation of immune genes by H3 acetylation in a strain-specific manner.
Related Results
Progress of Acetylation Modification in Plants
Progress of Acetylation Modification in Plants
Protein acetylation, a conserved post-translational modification, is collaboratively catalyzed by acetyltransferases and deacetylases and is widespread in plants. This study review...
Inhibition of Colon Cancer in Mice by Microencapsulated Probiotic
Inhibition of Colon Cancer in Mice by Microencapsulated Probiotic
Colon cancer is the third most common cancer worldwide with a high morbidity and mortality rate. Therapies are less effective during metastasis, therefore prevention and earlier de...
Cell-cycle-dependent repression of histone gene transcription by histone H4
Cell-cycle-dependent repression of histone gene transcription by histone H4
Abstract
In all eukaryotes DNA replication is coupled to histone synthesis to coordinate chromatin packaging of the genome. Canonical histone gen...
Temporal and spatial distribution of histone acetylation in mouse molar development
Temporal and spatial distribution of histone acetylation in mouse molar development
Histone acetylation is one of the most widely studied histone modification, regulating a variety of biological activities like organ development and tumorigenesis. However, the rol...
The Influence of Stationary and exponential Growth Phase of Probiotic Lactobacilli Towards Aggregatibacter actinomycetemcomitans Biofilm
The Influence of Stationary and exponential Growth Phase of Probiotic Lactobacilli Towards Aggregatibacter actinomycetemcomitans Biofilm
Background: Bacterial biofilm of the oral cavity contributes to the dispersion of pathogenic organisms to other organs, particularly in immunocompromised patients. Lactobacilli own...
Exploring Histone Acetylation in Ischemic Stroke: The Role of CREBBP and CKAP4 as Key Biomarkers
Exploring Histone Acetylation in Ischemic Stroke: The Role of CREBBP and CKAP4 as Key Biomarkers
Abstract
Background
Ischemic stroke (IS) is a serious cerebrovascular disease. Excessive acetylated protein levels are linked to neuronal resistance to ischemia, making hi...
Flame retardant tetrabromobisphenol A (TBBPA) disrupts histone acetylation during zebrafish maternal-to-zygotic transition
Flame retardant tetrabromobisphenol A (TBBPA) disrupts histone acetylation during zebrafish maternal-to-zygotic transition
ABSTRACT
3,3’,5.5’-Tetrabromobisphenol A (TBBPA) is a widely used brominated flame-retardant utilized in the production of electronic devices and plastic paints. Th...