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The structural origin of the color differences in the bioluminescence of firefly luciferase
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Six chimeric mutants between Hotaria parvula (λmax=568 nm) and Pyrocoelia miyako (λmax=550 nm) luciferases were reconstructed to determine the structural origin of the color differences in firefly luciferase. Based on light‐emitting color, five chimeric luciferases could be divided into two groups: the three green‐emitting mutants, classified as P. miyako luciferase, and the two yellow‐emitting mutants, classified as H. parvula luciferase. Their common fragments between Val‐209 and Ala‐318 within each group contain the active site for the color differences.
Title: The structural origin of the color differences in the bioluminescence of firefly luciferase
Description:
Six chimeric mutants between Hotaria parvula (λmax=568 nm) and Pyrocoelia miyako (λmax=550 nm) luciferases were reconstructed to determine the structural origin of the color differences in firefly luciferase.
Based on light‐emitting color, five chimeric luciferases could be divided into two groups: the three green‐emitting mutants, classified as P.
miyako luciferase, and the two yellow‐emitting mutants, classified as H.
parvula luciferase.
Their common fragments between Val‐209 and Ala‐318 within each group contain the active site for the color differences.
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